Human identification with STRs and SNPs

Simultaneously sequence multiple forensic STRs and SNPs in one test

Simplified SNP & STR Analysis

Many forensic labs currently utilize PCR and capillary electrophoresis (CE)-based methods to analyze short tandem repeat (STR) markers and other polymorphisms. A major limitation of CE typing is that multiple polymorphisms cannot be analyzed together, causing forensic labs to validate and maintain multiple PCR-based systems. CE typing can often necessitate multiple rounds of testing.

Next-generation sequencing (NGS) enables single nucleotide polymorphism (SNP) and STR analysis for large numbers of globally relevant STR markers and dense SNP sets in a single test. The accuracy and high-throughput of Illumina sequencing by synthesis (SBS) chemistry promises to streamline STR and SNP forensic lab operations.

Benefits of SNP & STR Analysis with NGS

  • STR sequencing can resolve alleles of identical size, but different sequence
  • High-throughput sequencing lets you simultaneously analyze multiple polymorphisms
  • Unlike CE typing, NGS does not require multiple forensic STR analysis kits
  • NGS-generated short tandem repeat analysis calls are fully compatible with current database formats, providing a seamless link between CE-based and NGS data

The Future of Forensic Genomics

Illumina technology helps researchers analyze forensically relevant STRs and SNPs.

SNP and STR Sequencing Workflow

Verogen offers a complete, fully validated DNA-to-data solution designed for forensic genomics. First, forensically relevant STRs and SNPs are simultaneously PCR amplified, with indexed adapters incorporated into each amplicon in a separate round of PCR.

Subsequent DNA cluster generation and sequencing by synthesis is performed on the MiSeq FGx Instrument. Finally, analyze and interpret your sequencing data off-line using ForenSeq Universal Analysis Software.

DNA Extraction & Quantitation

We recommend using your current DNA extraction and quantitation methods.

Library Preparation

ForenSeq DNA Signature Prep Kit (for nuclear DNA) Assay approximately 200 genetic markers in a single test. Includes all reagents to prepare sequencing libraries from forensic DNA samples.

MiSeq FGx Instrument
First fully validated next-generation sequencing system for forensic genomics.

MiSeq FGx Reagent Kit
Pre-filled, ready-to-use reagent cartridges, specifically designed for the MiSeq FGx System.

ForenSeq Universal Analysis Software
Full functionality to analyze and interpret a wide range of forensic casework and database samples.

Uses of SNP and STR Analysis

Criminal Casework

Targeted sequencing of forensically relevant STR and SNP loci empowers you to generate leads, even on cold cases. Learn More »

Forensic DNA Databases

NGS can help labs produce high-quality forensic profiles for national DNA databases, with a faster turnaround. Learn More »

Identifying Missing Persons

NGS delivers a single platform and workflow to overcome the wide range of difficult samples encountered in missing persons cases. Learn More »

Disaster Victim Identification

Genetic analysis of mass fatality evidence with NGS lets you extract as much information as possible from highly compromised samples. Learn More »

Additional Resources

Overview: NGS for Forensic Genomics

View an introduction to NGS and its applications for forensic genomics.

Take a Genomic Leap Forward in Forensic Science

The MiSeq FGx System is the first fully validated NGS solution designed exclusively for forensic science.

View Video

Harnessing NGS to Solve Crimes

Criminalists use MiSeq FGx for high-resolution DNA profiling to build better forensic DNA databases.

Recreating a Face Based on DNA

Researchers identify genetic variants that influence facial features to enable facial imaging from DNA.

Talk to a Forensic Expert

We create thoughtfully tailored genomic solutions for forensic DNA labs.
Let us help you overcome limitations and solve more cases.

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