Verogen Forms Strategic Partnership with QIAGEN Read the Press Release

ForenSeq MainstAY Kit

Target the core autosomal and Y-STRs with the ForenSeq MainstAY Kit, the most accessible way to generate highly discriminating data for mainstream casework and forensic genetic genealogy confirmatory testing. By focusing on established STRs used in routine human identification applications, MainstAY eases the transition to next-generation sequencing by maintaining compatibility with global databases. The familiar and proven ForenSeq workflow generates more comprehensive DNA profiles for up to 96 evidence samples with less than 2 hours of hands-on time.

The ForenSeq MainstAY Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). The kit is designed, developed, and manufactured as a high-performance end-to-end solution for forensic samples. Technical support is included for the entire portfolio, and quality-controlled manufacturing ensures reproducibility with the convenience of an all-in-one library prep kit.

Key Features

  • Focused and efficient analysis for volume casework Simultaneously interrogate the largest collection of well characterized loci including 27 autosomal STRs and 25 Y-STR markers for up to 96 samples. Generate balanced profiles compatible with all existing STR databases with as little as 100 pg. Efficiently analyze data with a minimal interaction analysis module and choose from a range of upload-ready export options compatible with regional database requirements.
  • Better data, similar cost Obtain highly discriminating data for core CODIS and European Standard Set (ESS) STR loci in a single amplification and sequencing run at a comparable cost to traditional CE analysis. Unlock the power of next-generation sequencing whatever your throughput requirement with a choice of two sequencing kit sizes.
  • Fully integrated and supported The end-to-end workflow is based on the established ForenSeq process, enabling a diverse range of DNA analysis on a single, proven platform. The ForenSeq MainstAY Kit links seamlessly with UAS for rapid data assessment and single-click report generation.


Sample type gDNA, buccal swabs, FTA card
Recommended input for gDNA 1 ng per sample
Recommended input for FTA card punch 1.2 mm per sample
Kit configuration 96 reaction kit and 384 reaction kit
Recommended multiplexing capacity 8 to 96 samples per run
Locus multiplexing capacity Simultaneous analysis of 52 genetic markers
Primer mix content 27 global autosomal STRs including CODIS and European Standard set
25 Y-STRs including minimum set for the Y Haplotype Reference Database (YHRD)
Amplicon size Mean: 235 bp
Maximum: 481 bp
Low-level mixture detection Detects minor contributors at 5%
Total library prep time 7 hours and 15 minutes
Hands-on library prep time 1 hour and 30 min
Total sequencing time ~28 hours with the MiSeq FGx Reagent Kit
~22 hours with the MiSeq FGx Reagent Micro Kit
Kit shelf life 1 year

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