Target the core autosomal and Y-STRs with the ForenSeq MainstAY Product Line, the most accessible way to generate highly discriminating data for mainstream casework and forensic genetic genealogy confirmatory testing. Choose between two geographically relevant options like the ForenSeq MainstAY kit, which is purpose-built to support a majority of national database STR uploads, or the ForenSeq MainstAY SE kit which includes the highly polymorphic SE33 marker. By focusing on established STRs used in routine human identification applications and maintaining compatibility with global databases, the MainstAY products ease the transition to next-generation sequencing. The familiar and proven ForenSeq workflow generates more comprehensive DNA profiles for up to 96 evidence samples with less than 2 hours of hands-on time.
The ForenSeq MainstAY Product Line is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). The kits are designed, developed, and manufactured as a high-performance end-to-end solution for forensic samples. Technical support is included for the entire portfolio, and quality-controlled manufacturing ensures reproducibility with the convenience of an all-in-one library prep kit.
Key Features
Specifications
| Sample type | extracted gDNA from a range of common forensic sample types, buccal swab lysate, FTA card |
| Recommended input for gDNA | 1 ng per sample |
| Recommended input for FTA card punch | 1.2 mm per sample |
| Kit options |
ForenSeq MainstAY kit ForenSeq MainstAY SE kit |
| Kit configuration |
ForenSeq MainstAY: 96 reaction kit and 384 reaction kit ForenSeq MainstAY SE: 96 reaction kit |
| Recommended multiplexing capacity | 8 to 96 samples per run |
| Locus multiplexing capacity | Simultaneous analysis of 52 genetic markers |
| Primer mix content |
ForenSeq MainstAY Kit: 27 global autosomal STRs including CODIS and European Standard set 25 Y-STRs including minimum set for the Y Haplotype Reference Database (YHRD) ForenSeq MainstAY SE Kit: Includes all markers in ForenSeq MainstAY Kit + SE33 |
| Amplicon size |
Mean: 235 bp Maximum: 481 bp |
| Low-level mixture detection | Detects minor contributors at 5% |
| Total library prep time | 7 hours and 15 minutes |
| Hands-on library prep time | 1 hour and 30 min |
| Total sequencing time | ~22 hours with the MiSeq FGx Reagent Micro Kit |
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