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ForenSeq MainstAY Product Line

Target the core autosomal and Y-STRs with the ForenSeq MainstAY Product Line, the most accessible way to generate highly discriminating data for mainstream casework and forensic genetic genealogy confirmatory testing. Choose between two geographically relevant options like the ForenSeq MainstAY kit, which is purpose-built to support a majority of national database STR uploads, or the ForenSeq MainstAY SE kit which includes the highly polymorphic SE33 marker. By focusing on established STRs used in routine human identification applications and maintaining compatibility with global databases, the MainstAY products ease the transition to next-generation sequencing. The familiar and proven ForenSeq workflow generates more comprehensive DNA profiles for up to 96 evidence samples with less than 2 hours of hands-on time.

The ForenSeq MainstAY Product Line is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). The kits are designed, developed, and manufactured as a high-performance end-to-end solution for forensic samples. Technical support is included for the entire portfolio, and quality-controlled manufacturing ensures reproducibility with the convenience of an all-in-one library prep kit.

Key Features


  • Focused and efficient analysis for volume casework Simultaneously interrogate the largest collection of well characterized loci for up to 96 samples. Interrogate 27 core autosomal STRs and 25 Y-STR markers, with flexible options to evaluate SE33. Generate balanced profiles compatible with all existing STR databases with as little as 100 pg. Efficiently analyze data with a minimal interaction analysis module and choose from a range of upload-ready export options compatible with regional database requirements. Efficiently analyze data with a minimal interaction analysis module and choose from a range of upload-ready export options compatible with regional database requirements.
  • Better data, similar cost Obtain highly discriminating data for core CODIS, German and European Standard Set (ESS) STR loci in a single amplification and sequencing run at a comparable cost to traditional CE analysis. Unlock the power of next-generation sequencing with choice of marker content and sequencing kit sizes to match your throughput requirements. Unlock the power of next-generation sequencing whatever your throughput requirement with a choice of two sequencing kit sizes.
  • Fully integrated and supported The end-to-end workflow is based on the established ForenSeq process, enabling a diverse range of DNA analysis on a single, proven platform. The ForenSeq MainstAY Kit and ForenSeq MainstAY SE Kit links seamlessly with UAS for rapid data assessment and single-click report generation.

Specifications


Sample type extracted gDNA from a range of common forensic sample types, buccal swab lysate, FTA card
Recommended input for gDNA 1 ng per sample
Recommended input for FTA card punch 1.2 mm per sample
Kit options ForenSeq MainstAY kit
ForenSeq MainstAY SE kit
Kit configuration ForenSeq MainstAY: 96 reaction kit and 384 reaction kit
ForenSeq MainstAY SE: 96 reaction kit
Recommended multiplexing capacity 8 to 96 samples per run
Locus multiplexing capacity Simultaneous analysis of 52 genetic markers
Primer mix content ForenSeq MainstAY Kit: 27 global autosomal STRs including CODIS and European Standard set 25 Y-STRs including minimum set for the Y Haplotype Reference Database (YHRD)
ForenSeq MainstAY SE Kit: Includes all markers in ForenSeq MainstAY Kit + SE33
Amplicon size Mean: 235 bp
Maximum: 481 bp
Low-level mixture detection Detects minor contributors at 5%
Total library prep time 7 hours and 15 minutes
Hands-on library prep time 1 hour and 30 min
Total sequencing time ~22 hours with the MiSeq FGx Reagent Micro Kit

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