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Target the core autosomal and Y-STRs with the ForenSeq MainstAY Kit, the most accessible way to generate highly discriminating data for mainstream casework and forensic genetic genealogy confirmatory testing. By focusing on established STRs used in routine human identification applications, MainstAY eases the transition to next-generation sequencing by maintaining compatibility with global databases. The familiar and proven ForenSeq workflow generates more comprehensive DNA profiles for up to 96 evidence samples with less than 2 hours of hands-on time.
The ForenSeq MainstAY Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). The kit is designed, developed, and manufactured as a high-performance end-to-end solution for forensic samples. Technical support is included for the entire portfolio, and quality-controlled manufacturing ensures reproducibility with the convenience of an all-in-one library prep kit.
Key Features
Specifications
| Sample type | gDNA, buccal swabs, FTA card |
| Recommended input for gDNA | 1 ng per sample |
| Recommended input for FTA card punch | 1.2 mm per sample |
| Kit configuration | 96 reaction kit and 384 reaction kit |
| Recommended multiplexing capacity | 8 to 96 samples per run |
| Locus multiplexing capacity | Simultaneous analysis of 52 genetic markers |
| Primer mix content |
27 global autosomal STRs including CODIS and European Standard set 25 Y-STRs including minimum set for the Y Haplotype Reference Database (YHRD) |
| Amplicon size |
Mean: 235 bp Maximum: 481 bp |
| Low-level mixture detection | Detects minor contributors at 5% |
| Total library prep time | 7 hours and 15 minutes |
| Hands-on library prep time | 1 hour and 30 min |
| Total sequencing time |
~28 hours with the MiSeq FGx Reagent Kit ~22 hours with the MiSeq FGx Reagent Micro Kit |
| Kit shelf life | 1 year |
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