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ForenSeq mtDNA Whole Genome Kit

The ForenSeq mtDNA Whole Genome Kit offers a convenient and cost-effective library prep for sequencing the whole mitochondrial genome (mtGenome) with minimal DNA input and exceptional data recovery. Leveraging proven ForenSeq chemistry, the kit produces expanded data sets that promote population studies or provide additional resolution for severely degraded samples. Moreover, this solution has the lowest list price of any commercial whole genome assay. Multiplexing capability further boosts operational efficiency without increases to costs or time.

The ForenSeq mtDNA Whole Genome Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as an end-to-end solution for high performance with forensic samples, extensive technical support backs the entire portfolio. Quality-controlled reagent manufacturing ensures reproducibility with the convenience of all-in-one kitting.

Key Features


  • Reliable Results The targeted PCR-based assay deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection. A tiled primer approach overlaps the amplicons to prevent the sequence gaps that can lead to data loss, particularly in degraded samples. Complementing this tiled primer design, an enhanced buffer system delivers exceptional resistance to calcium, humic acid, and other relevant PCR inhibitors.
  • Flexible Workflow Options The protocol includes two normalization methods to tailor library prep for a broad range of input material, from high-quality genomic DNA (gDNA) through low-level and complex samples. A low input requirement expands possibilities for the most challenging samples. For efficient use of the reagent kit and accurate read assignment, index adapters provided in the library prep kit allow pooling and sequencing of up to 16 samples in one run.
  • Complex Bioinformatics, Simplified The ForenSeq mtDNA Whole Genome Kit pairs seamlessly with the ForenSeq mtDNA Analysis Module in UAS for ease-of-use and rapid review of mtDNA data. The software analyzes sequencing output for the whole mitochondrial genome and provides results less than one hour after run completion. Laboratories can review summarized or detailed results and compare up to nine samples on a clear and interactive user interface. Reports are compatible with multiple DNA databases.

Specifications


Target size 16,569 bp mtGenome
Sample type gDNA and mtDNA extracted from hair, bone, teeth, and buccal swabs
DNA input recommendation 100 pg gDNA per sample
Kit configuration 48 reactions
Multiplexing capacity 16 libraries per run
Number of primers > 500
Number of amplicons 245
Average amplicon size 131 bp
Amplicon overlap ≥ 3 bp
Total library prep time 7 hours and 45 minutes
Hands-on time 1 hour and 45 minutes

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