The ForenSeq mtDNA Whole Genome Kit offers a convenient and cost-effective library prep for sequencing the whole mitochondrial genome (mtGenome) with minimal DNA input and exceptional data recovery. Leveraging proven ForenSeq chemistry, the kit produces expanded data sets that promote population studies or provide additional resolution for severely degraded samples. Moreover, this solution has the lowest list price of any commercial whole genome assay. Multiplexing capability further boosts operational efficiency without increases to costs or time.
The ForenSeq mtDNA Whole Genome Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as an end-to-end solution for high performance with forensic samples, extensive technical support backs the entire portfolio. Quality-controlled reagent manufacturing ensures reproducibility with the convenience of all-in-one kitting.
|Target size||16,569 bp mtGenome|
|Sample type||gDNA and mtDNA extracted from hair, bone, teeth, and buccal swabs|
|DNA input recommendation||100 pg gDNA per sample|
|Kit configuration||48 reactions|
|Multiplexing capacity||16 libraries per run|
|Number of primers||> 500|
|Number of amplicons||245|
|Average amplicon size||131 bp|
|Amplicon overlap||≥ 3 bp|
|Total library prep time||7 hours and 45 minutes|
|Hands-on time||1 hour and 45 minutes|