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ForenSeq DNA Signature Prep Kit

 

Generate more comprehensive DNA profiles for up to 96 DNA samples with fewer than two hours of hands-on time using our simple, plate-based workflow. The ForenSeq DNA Signature Prep Kit is the only next-generation sequencing (NGS)-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework. Combining over 200 markers, including autosomal, X- and Y-STRs, identity-informative and phenotypic-informative SNPs into a single, streamlined workflow, the kit eliminates the need to run multiple STR tests.

The ForenSeq DNA Signature Prep Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx System and analyzes data in ForenSeq Universal Analysis Software (UAS). Designed, developed, and manufactured as a high performance, end-to-end solution for forensic samples, the entire portfolio is backed by extensive technical support. In addition, quality-controlled manufacturing ensures reproducibility with the convenience of all-in-one library prep kits.

verogen prep kit boxes

Superior Analysis of Challenging Samples

Recover more highly discriminating genetic information from heavily degraded DNA than traditional methods with a high concentration of amplicon sizes less than 200 bp.

Increased Resolution for Mixtures

Leverage the power and sensitivity of the MiSeq FGx System to detect minor contributors at < 5% in mixed samples.

No Need for Multiple Kits

Simultaneously interrogate your choice of markers. Choose from either DNA Primer Set A (DPMA) or DNA Primer Set B (DPMB) to consolidate multiple existing capillary electrophoresis (CE)-based kits and meet all your marker needs in one amplification.

Specifications

Sample type gDNA, buccal swabs, FTA card
Recommended input for gDNA 1 ng per sample
Recommended input for FTA card punch 1.2 mm per sample
Multiplexing capacity 8–96 libraries per run
Locus multiplexing capability Simultaneous analysis of ~200 genetic markers
Number of primer mixes 2 (DPMA and DPMB)
Primer mix content

DPMA: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs

DPMB: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs, 22 phenotypic SNPs, 56 biogeographical ancestry SNPs

Short amplicon detection High concentration of markers < 200 bp
Low-level mixture detection Detects minor contributors at < 5%
Total library prep time 7 hours and 15 minutes
Hands-on time 1 hour and 45 minutes
Total sequencing time

~30 hours with the MiSeq FGx Reagent Kit

~22 hours with the MiSeq FGx Reagent Micro Kit

Resources

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