ForenSeq DNA Signature Prep Kit
Generate more comprehensive DNA profiles for up to 96 DNA samples with fewer than two hours of hands-on time using our simple, plate-based workflow. The ForenSeq DNA Signature Prep Kit is the only next-generation sequencing (NGS)-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework. Combining over 200 markers, including autosomal, X- and Y-STRs, identity-informative and phenotypic-informative SNPs into a single, streamlined workflow, the kit eliminates the need to run multiple STR tests.
The ForenSeq DNA Signature Prep Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx System and analyzes data in ForenSeq Universal Analysis Software (UAS). Designed, developed, and manufactured as a high performance, end-to-end solution for forensic samples, the entire portfolio is backed by extensive technical support. In addition, quality-controlled manufacturing ensures reproducibility with the convenience of all-in-one library prep kits.
Superior Analysis of Challenging Samples
Recover more highly discriminating genetic information from heavily degraded DNA than traditional methods with a high concentration of amplicon sizes less than 200 bp.
Increased Resolution for Mixtures
Leverage the power and sensitivity of the MiSeq FGx System to detect minor contributors at < 5% in mixed samples.
No Need for Multiple Kits
Simultaneously interrogate your choice of markers. Choose from either DNA Primer Set A (DPMA) or DNA Primer Set B (DPMB) to consolidate multiple existing capillary electrophoresis (CE)-based kits and meet all your marker needs in one amplification.
Specifications
| Sample type | gDNA, buccal swabs, FTA card |
| Recommended input for gDNA | 1 ng per sample |
| Recommended input for FTA card punch | 1.2 mm per sample |
| Multiplexing capacity | 8–96 libraries per run |
| Locus multiplexing capability | Simultaneous analysis of ~200 genetic markers |
| Number of primer mixes | 2 (DPMA and DPMB) |
| Primer mix content |
DPMA: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs DPMB: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs, 22 phenotypic SNPs, 56 biogeographical ancestry SNPs |
| Short amplicon detection | High concentration of markers < 200 bp |
| Low-level mixture detection | Detects minor contributors at < 5% |
| Total library prep time | 7 hours and 15 minutes |
| Hands-on time | 1 hour and 45 minutes |
| Total sequencing time |
~30 hours with the MiSeq FGx Reagent Kit ~22 hours with the MiSeq FGx Reagent Micro Kit |
Resources
Related Products
MiSeq FGx System
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ForenSeq Universal Analysis Software
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