Generate more comprehensive DNA profiles for up to 96 DNA samples with fewer than two hours of hands-on time using our simple, plate-based workflow. The ForenSeq DNA Signature Prep Kit is the first next-generation sequencing (NGS)-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework. Combining over 200 markers, including autosomal, X- and Y-STRs, identity-informative, phenotype-informative and biogeographical ancestry-informative SNPs into a single, streamlined workflow, the kit eliminates the need to run multiple STR tests.
The ForenSeq DNA Signature Prep Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as a high performance, end-to-end solution for forensic samples, the entire portfolio is backed by extensive technical support. In addition, quality-controlled manufacturing ensures reproducibility with the convenience of all-in-one library prep kits.
Key Features
Specifications
| Sample type | extracted gDNA from a range of common forensic sample types, buccal swab lysate, FTA card |
| Recommended input for gDNA | 1 ng per sample |
| Recommended input for FTA card punch | 1.2 mm per sample |
| Recommended multiplexing capacity | 8–96 libraries per run |
| Locus multiplexing capability | Simultaneous analysis of ~200 genetic markers |
| Number of primer mixes | 2 (DPMA and DPMB) |
| Primer mix content |
DPMA: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs DPMB: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs, 22 phenotypic SNPs, 56 biogeographical ancestry SNPs |
| Short amplicon detection | High concentration of markers < 200 bp |
| Low-level mixture detection | Detects minor contributors at < 5% |
| Total library prep time | 7 hours and 15 minutes |
| Hands-on time | 1 hour and 45 minutes |
| Total sequencing time |
~28 hours with the MiSeq FGx Reagent Kit ~22 hours with the MiSeq FGx Reagent Micro Kit |
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