ForenSeq Enhanced PCR1 Buffer System

The ForenSeq Enhanced PCR1 Buffer System is a cost-effective solution that offers superior recovery of DNA from inhibited samples. Optimized to work with severely compromised samples such as degraded skeletal remains, this kit can be used with both the ForenSeq DNA Signature Prep and the ForenSeq MainstAY workflows to produce high-quality DNA profiles. A low cost per sample enables easy integration into both workflows further boosting operational efficiency without increases to overall turnaround time.

The ForenSeq Enhanced PCR1 Buffer System is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as a modular solution supporting an end-to-end workflow for challenging forensic samples, extensive technical support backs the entire portfolio. Quality-controlled reagent manufacturing ensures reproducibility with the convenience of all-in-one kitting.

Key Features


  • Superior Results This high-performance buffer has been designed and developed to enhance DNA profile recovery with casework-type samples. Exceptional resistance to high concentrations of hematin, tannic acid and humic acid make it optimal for severely inhibited samples such as degraded interred bones.This buffer system includes protocol enhancements that deliver a 5-fold improvement in DNA profile recovery when used in conjunction with the ForenSeq DNA Signature Prep Kit, and 5 to 50 fold improvement with the ForenSeq MainstAY Kit.
  • Flexible Workflow Options Compatible with two Verogen products – the ForenSeq MainstAY and ForenSeq DNA Signature Prep Kits, labs can benefit from superior DNA recovery regardless of choice of workflow. A low input requirement expands possibilities for the most challenging samples. Tailored for all inhibited samples and optimized for two reagent kits, this workflow can be easily added to or swapped into mainstream and niche workflows.
  • Simplified Implementation For labs that with validated workflows, the ForenSeq Enhanced PCR1 Buffer pairs seamlessly with the ForenSeq MainstAY and ForenSeq DNA Signature Prep Kits. Engineered as a minor protocol enhancement, this buffer system can be included into validated workflows without the need for revalidation. Verogen guidance and support enables easy integration of this buffer into existing workflows with minimal onboarding burden.

Specifications


Sample type extracted gDNA from a range of common forensic sample types
DNA input recommendation 1ng per sample
Compatibility ForenSeq MainstAY Kit
ForenSeq DNA Signature Prep Kit
Kit configuration 96 reactions
Multiplexing capacity for the ForenSeq MainstAY Kit 96 libraries per run on a MiSeq FGx Reagent Micro Kit
Multiplexing capacity for ForenSeq DNA Signature Prep Kit
(Primer Mix A)
36/96 database and 12/32 casework libraries per run on a MiSeq FGx Micro/Standard Kit respectively
Multiplexing capacity for ForenSeq DNA Signature Prep Kit
(Primer Mix B)
12/32 libraries per run on a MiSeq FGx Micro/Standard Kit respectively
Total library prep time ForenSeq MainstAY Kit: 7 hours and 15 minutes
ForenSeq DNA Signature Prep Kit: 7 hours and 15 minutes
Hands-on time ForenSeq MainstAY Kit: 1 hour and 30 minutes
ForenSeq DNA Signature Prep Kit: 1 hour and 30 minutes

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