Target 10,230 SNP markers explicitly curated for forensic kinship use with the ForenSeq Kintelligence Kit. The only sequencing-based assay designed for forensic genetic genealogy (FGG), ForenSeq Kintelligence minimizes privacy concerns by excluding medically informative and minor allele frequency SNPs found in whole-genome sequencing (WGS) or array-based workflows. Robust PCR and primer designs allow for a single SNP multiplex that works across global populations.
The ForenSeq Kintelligence Kit is part of an integrated and supported workflow designed, developed, and validated to deliver high-quality outcomes with low-quality samples. Prepare up to 12 forensic samples per ForenSeq Kintelligence Kit. Sequence up to three samples per MiSeq FGx System run according to predefined run protocols. Analyze data in Universal Analysis Software (UAS) using the preconfigured ForenSeq Kintelligence Analysis Module, which includes recommended thresholds based on extensive in-house testing. Generate a GEDmatch PRO compatible report in less than one hour for investigative lead generation.
|Sample type||extracted gDNA from a range of common forensic sample types|
|DNA input recommendation||1 ng per sample|
|Kit configuration||12 reactions|
|Recommended multiplexing capacity||3 libraries per run|
|Number of SNPs||10,230|
|Mean amplicon size||< 150 bp|
|Total library prep time||8 hours|
|Hands-on library prep time||1 hour and 55 minutes|
|Total sequencing time||~28 hours with the MiSeq FGx Reagent Kit|