Eliminate trade-offs in disaster victim identification

Disaster Victim Identification

Introduction to Disaster Victim Identification

Genetic analysis can help identify victims of mass fatalities when traditional methods cannot be used, or fail to yield conclusive results. Because next-generation sequencing (NGS) has no relevant limit on the number of loci that can be simultaneously analyzed, you can extract the maximum amount of information from evidence or a known reference sample.

Conventional disaster victim identification methods include fingerprints and odontology. Labs also use molecular methods such as DNA analysis and mitochondrial DNA (mtDNA) sequencing with capillary electrophoresis. Sample types processed after a mass fatality event can include bone fragments, tissue, various personal effects containing DNA, and buccal swabs from relatives.

The goal of DNA testing for disaster victim identification is to extract as much genetic information as possible from highly compromised samples. With traditional DNA testing using capillary electrophoresis (CE), choosing one assay often precludes the ability to perform another. Analysts must evaluate the evidentiary material and decide which assay method to perform.

With Verogen NGS technology, these tradeoffs are eliminated. The MiSeq FGx Forensic Genomics System has no limit on the number of loci that can be analyzed on the same sample in the same run. Every relevant locus can be assayed, including core sets of autosomal, Y and X STRs, and several categories of SNPs. The mtDNA control region and genome can also be analyzed using the same efficient workflow and analysis software.

Workflow for Disaster Victim Identification

For disaster victim identification, Verogen offers a complete, fully validated DNA-to-data solution designed for forensic genomics. Our recommended NGS workflow includes a dedicated library prep kit, customized benchtop sequencing instrument, and an analytical software package to simplify your lab operations.

Verogen sequencing by synthesis (SBS) technology on the MiSeq FGx Forensic Genomics System combines multiple tests in a single analysis. Achieve high resolution and exceptional accuracy from minute amounts of DNA—even with complex mixtures or degraded DNA.

Library Preparation:

ForenSeq DNA Signature Prep Kit (for nuclear DNA) Assay approximately 200 genetic markers in a single test. Includes all reagents to prepare sequencing libraries from forensic DNA samples.

ForenSeq mtDNA Control Region Kit Analyze the mitochondrial DNA control region from high-quality or degraded samples.

ForenSeq mtDNA Whole Genome Kit A fully integrated, Sample-to-result mitochondrial solution.

MiSeq FGx Instrument

First fully validated sequencing system for forensic genomics.

MiSeq FGx Reagent Kit

Pre-filled, ready-to-use reagent cartridges, specifically designed for the MiSeq FGx System.

ForenSeq Universal Analysis Software

Analyze and interpret a wide range of forensics casework and database samples.

Validated Solutions for Disaster Victim Identification

SNP & STR Analysis with NGS

NGS enables genetic analysis for large numbers of globally relevant STR markers and dense SNP sets in a single test. Learn More »

Forensic mtDNA Sequencing

NGS provides deep coverage of mtDNA to extract genetic data from small forensic samples found in less than ideal condition. Learn More »

Additional Resources

mtDNA Sequencing to ID Skeletal Remains

Dr. Mitch Holland of Penn State explains how high-throughput sequencing is helping us realize the power of mtDNA heteroplasmy in forensic research.

View Video

Application Note

A Comprehensive Next-Generation Sequencing Workflow for Severely Degraded Nuclear DNA

Data Sheet

ForenSeq™ mtDNA Control Region Solution Data Sheet

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