ForenSeq DNA Signature Prep Kit

Generate more comprehensive DNA profiles for up to 96 DNA samples with fewer than two hours of hands-on time using our simple, plate-based workflow. The ForenSeq DNA Signature Prep Kit is the first next-generation sequencing (NGS)-based short-tandem repeat (STR) sequencing chemistry approved for upload to the National DNA Index System (NDIS) for casework. Combining over 200 markers, including autosomal, X- and Y-STRs, identity-informative, phenotype-informative and biogeographical ancestry-informative SNPs into a single, streamlined workflow, the kit eliminates the need to run multiple STR tests.

The ForenSeq DNA Signature Prep Kit is part of an integrated workflow that sequences libraries on the MiSeq FGx Sequencing System and analyzes data in Universal Analysis Software (UAS). Designed, developed, and manufactured as a high performance, end-to-end solution for forensic samples, the entire portfolio is backed by extensive technical support. In addition, quality-controlled manufacturing ensures reproducibility with the convenience of all-in-one library prep kits.

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Key Features

Superior Analysis of Challenging Samples Recover more highly discriminating genetic information from heavily degraded DNA than traditional methods with a high concentration of amplicon sizes less than 200 bp.
Increased Resolution for Mixtures Leverage the power and sensitivity of the MiSeq FGx System to detect minor contributors at < 5% in mixed samples.
No Need for Multiple Kits Simultaneously interrogate your choice of markers in a single amplification. Choose from either DNA Primer Set A (DPMA) or DNA Primer Set B (DPMB) to consolidate multiple existing capillary electrophoresis (CE)-based kits.

Specifications

Sample type	extracted gDNA from a range of common forensic sample types, buccal swab lysate, FTA card
Recommended input for gDNA	1 ng per sample
Recommended input for FTA card punch	1.2 mm per sample
Recommended multiplexing capacity	8–96 libraries per run
Locus multiplexing capability	Simultaneous analysis of ~200 genetic markers
Number of primer mixes	2 (DPMA and DPMB)
Primer mix content	DPMA: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs DPMB: 27 global autosomal STRs, 24 Y-STRs, 7 X-STRs, 94 identity SNPs, 22 phenotypic SNPs, 56 biogeographical ancestry SNPs
Short amplicon detection	High concentration of markers < 200 bp
Low-level mixture detection	Detects minor contributors at < 5%
Total library prep time	7 hours and 15 minutes
Hands-on time	1 hour and 45 minutes
Total sequencing time	~28 hours with the MiSeq FGx Reagent Kit ~22 hours with the MiSeq FGx Reagent Micro Kit