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Learn more about popular topics and find resources that will help you with all of your Verogen products.

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Technical Resources
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Technical Resources

TitleProductsResource TypesDownloadproducts_hfilterresource_types_hfilter
MiSeq FGx™ System Overview BrochureMiSeq FGx™Brochuresmiseq-fgxbrochures
ForenSeq™ DNA Signature Prep Reference GuideForenSeq™ DNA Signature Prep KitReference Guidesforenseq-dna-signature-prep-kitreference-guides
ForenSeq™ Universal Analysis Software – Software GuideForenSeq™ Universal Analysis SoftwareReference Guidesforenseq-universal-analysis-softwarereference-guides
Human mtDNA Whole Genome GuidemtDNA AnalysisReference Guidesmtdna-analysisreference-guides
Human mtDNA D-Loop GuidemtDNA AnalysisReference Guidesmtdna-analysisreference-guides
mtDNA Variant Processor Application GuidemtDNA AnalysisApplication Guidesmtdna-analysisapplication-guides
mtDNA Variant Analyzer Application GuidemtDNA AnalysisApplication Guidesmtdna-analysisapplication-guides
Nextera XT DNA Library Preparation Kit Data SheetmtDNA AnalysisData Sheetsmtdna-analysisdata-sheets
Nextera Library Prep Application NotemtDNA AnalysisApplication Notesmtdna-analysisapplication-notes
Improved mtDNA Workflow PostermtDNA AnalysisPostersmtdna-analysisposters
ForenSeq™ DNA Materials ListForenSeq™ DNA Signature Prep KitSupporting Documentationforenseq-dna-signature-prep-kitsupporting-documentation
ForenSeq™ DNA Signature Prep Kit Check ListForenSeq™ DNA Signature Prep KitCheck Listsforenseq-dna-signature-prep-kitcheck-lists
ForenSeq™ DNA Signature Prep Kit Data SheetForenSeq™ DNA Signature Prep KitData Sheetsforenseq-dna-signature-prep-kitdata-sheets
MiSeq FGx™ Sequencer Safety & Compliance GuideMiSeq FGx™Safety and Compliance Guidesmiseq-fgxsafety-and-compliance-guides
MiSeq FGx™ Sequencer Site Prep GuideMiSeq FGx™Site Prep Guidesmiseq-fgxsite-prep-guides
MiSeq FGx™ System Data SheetMiSeq FGx™Data Sheetsmiseq-fgxdata-sheets
Nextera XT DNA Library Preparation Kit Data SheetmtDNA AnalysisData Sheetsmtdna-analysisdata-sheets
A Comprehensive Next-Generation Sequencing Workflow for Severely Degraded Nuclear DNAGeneralApplication Notesgeneralapplication-notes
Targeted NGS for Forensic GenomicsGeneralApplication Notesgeneralapplication-notes
Harnessing NGS to Solve CrimesGeneralCase Studies-Interviewsgeneralcase-studies-interviews
Recreating a Face Based on DNAGeneralCase Studies-Interviewsgeneralcase-studies-interviews
The Future of Forensic Genomics is HereGeneralCase Studies-Interviewsgeneralcase-studies-interviews
MiSeq FGx™ Reagent Micro Kit Technical NoteMiSeq FGx™Technical Notemiseq-fgxtechnical-note
ForenSeq™ Universal Analysis Software Data SheetForenSeq™ Universal Analysis SoftwareData Sheetsforenseq-universal-analysis-softwaredata-sheets
Analysis of Skeletal RemainsmtDNA AnalysisApplication Notesmtdna-analysisapplication-notes
MiSeq FGx™ Sequencer Reference GuideMiSeq FGx™Reference Guidesmiseq-fgxreference-guides

Knowledge Center

Experience the Technology of the MiSeq FGx System
Massively parallel sequencing (MPS) has become increasingly popular among forensic genomics laboratories, but for many labs, adopting a new technology like MPS may seem like a challenge. Find out how our two-day workshops can offer you the opportunity to gain practical experience with the MiSeq FGx Platform and provide you with the expertise to implement an MPS workflow in your lab.
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Bioinformatics at Work in the MiSeq FGx System
A guide to the robust bioinformatics and analytical capabilities of the ForenSeq Universal Analysis Software.
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Making the MPS Vision a Reality
Massively parallel sequencing (MPS) has become increasingly popular among forensic genomics laboratories. However, there are some challenges to implementation. Experts in the field of forensic genomics address these concerns and discuss the many benefits of MPS in everyday casework.
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Take a Genomic Leap Forward in Forensic Science
The MiSeq FGx System is the first fully validated NGS solution designed exclusively for forensic science.
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Massively Parallel Sequencing – The Time is Now
Hear from experts in the field of forensic genomics how this technology is paving the way ahead and why the time is now to embrace this technology.
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Introduction to Sequencing by Synthesis (SBS)
Learn about the sequencing by synthesis (SBS) technology employed on the MiSeq FGx, in 3-dimensional detail.
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The Power of mtDNA Heteroplasmy
Dr. Mitch Holland of Penn State explains how NGS is helping us realize the power of mtDNA heteroplasmy.
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Developmental Validation of the MiSeq FGx Forensic Genomics System for Targeted Next Generation Sequencing in Forensic DNA Casework and Database Laboratories Summary: The developmental validation of the MiSeq FGx Forensic Genomics System was conducted according to the 2012 SWGDAM Validation Guidelines for DNA Analysis Methods. Results demonstrated that the performance is accurate, robust, sensitive, specific, reliable, and reproducible on samples of various quality. View Online


Massively parallel sequencing of forensic STRs and SNPs using the Illumina® ForenSeq™ DNA Signature Prep Kit on the MiSeq FGx™ Forensic Genomics System Summary: A highly detailed investigation of the MiSeq FGx Forensic Genomics System across three fundamental vectors: methodological optimization, sequencing performance, and the ForenSeq kit characteristics.  The results mirror closely the performance reported in the developmental validation leading the authors to conclude, “In general, the ForenSeq DNA Signature Prep Kit is a well-performed, robust, reliable, reproducible and highly informative assay, and it is fully capable of human identification not only for single purified genomic DNA but also for LCN, mixture, inhibited, degraded and case-type samples.” View Online


Population and performance analyses of four major populations with Illumina’s FGx Forensic Genomics System Summary: An extensive study of the MiSeq FGx Forensic Genomics System was performed on 725 samples spanning four major population groups.  The implications of combining the power of both STRs and identity SNPs for random match probability calculations were investigated.  The use of sequence alleles, in addition to length alleles, further improved discrimination power. View Online


Automation of library preparation using Illumina ForenSeq kit for routine sequencing of casework samples Summary: The Laboratoire de Police Scientifique de Lyon worked with Hamilton Bonaduz to automate the sample preparation of the ForenSeq Signature Prep Kit on a Hamilton NGS STARlet HID platform.  The impressive results of their validation as well as the benefits of the automated approach are presented.  Their success demonstrates an efficient pathway for integration of the MiSeq FGx Forensic Genomics System into casework workflows globally. View Online


 A preliminary assessment of the ForenSeq™ FGx System: next generation sequencing of an STR and SNP multiplex Summary: The authors performed a rigorous evaluation of the MiSeq FGx Forensic Genomics System according to the guidelines described by SWGDAM for an internal validation.  Results obtained were checked for concordance and accuracy against orthogonal approaches (i.e. CE, FROG-kb, 23andMe) and the system was found to perform well.  The work presented here is especially useful for operational laboratories planning internal validation experiments. View Online


Increasing the reach of forensic genetics with massively parallel sequencing Summary: There is massive potential for applications of massively parallel sequencing (MPS) in forensic genomics.  This review acknowledges the proven technology and describes near-term opportunities to leverage the technology in targeting today’s problems. Specifically, molecular autopsy, microbial forensics, and the differentiation of monozygotic twins is approached and discussed through the lens of what is possible and practical in today’s environment. View Online


 Performance and concordance of the ForenSeq™ system for autosomal and Y chromosome short tandem repeat sequencing of reference-type specimens Summary: The MiSeq FGx Forensic Genomics System was evaluated by the Federal Bureau of Investigation for its applicability to analyzing reference samples.  In particular, an extensive evaluation of the data analysis was conducted to elucidate the impact of multiplex size and read counts on data recovery and concordance.  This foundational work further inspects the ability of the ForenSeq Universal Analysis Software to effectively flag low-quality or ambiguous results.  The results led the authors to conclude, “for high volume aSTR or Y-STR sequencing, such as in population studies or databasing of known specimens, it may be feasible to use the UAS as an expert system and limit analyst review to only those loci with quality indicators.” View Online

Targeted NGS for Forensic Genomics | Learn how Verogen next-generation sequencing (NGS) works, and its applications for forensic genomics. Read Note

A Comprehensive Next-Generation Sequencing Workflow for Severely Degraded Nuclear DNA Read Note

Analysis of Skeletal Remains | Find out how deep mitochondrial DNA (mtDNA) sequencing is being used to identify skeletal remains. Read Note

Developmental Validation of NGS | Learn about the advantages of targeted NGS and view the data validating the MiSeq FGx System in this Forensic Magazine article. Read Article

Analyzing Unknown Human Remains | Learn about the potential of NGS to solve cold missing persons cases and identify unknown human remains. Read Article

NGS Overcomes Limitations in Forensic Genomics | In forensic work, the smallest detail can make the difference in solving a crime. Read Article

The Future of Forensic Genomics is Here | King’s College London researchers are finding that the MiSeq FGx™ Forensic Genomics System provides more information-rich data than CE-based forensic DNA testing methods. Read Study

Harnessing NGS to Solve Crimes | Criminalists at the California Department of Justice find that the MiSeq FGx™ Forensic Genomics System is a significant advancement in DNA profiling technology over traditional CE-based methods. Read Study

Recreating a Face Based on DNA | Researchers identify genetic variants that influence facial features to enable facial imaging from DNA. Read Study

MiSeq FGx™ System Demonstration | Experience the simplicity of the MiSeq FGx™ System for forensic genomics View the Webcast

Developmental Validation of the MiSeq FGx™ System | Learn about the performance of the ForenSeq™ DNA Signature Prep Kit and ForenSeq™ Universal Analysis Software as well as comparisons to orthogonal SNP and STR allele calling methods View the Webcast

Forensic Genomics Webinar | Learn how the Verogen forensic genomics workflow can transform forensic applications View the Webcast

Forensic Science Technology Center of Excellence MPS Webinar Series: MPS I | Understanding the Basic Technology View the Webcast

Forensic Science Technology Center of Excellence MPS Webinar Series: MPS II | The Genetic Marker Potential View the Webcast

Forensic Science Technology Center of Excellence MPS Webinar Series: MPS III | Bioinformatics View the Webcast

Forensic Science Technology Center of Excellence MPS Webinar Series: MPS IV | Bioinformatics View the Webcast

Forensic Science Technology Center of Excellence MPS Webinar Series | Legal Perspective on the Adoption of Advanced Technologies View the Webcast

Contact Us

Have a question for our forensic genomics application experts? Need support or want to request a quote or free software demo? Please fill out this brief form and we will respond as soon as possible.

You can also call us at 1.833.837.6436 toll-free (North America) or 1.858.285.4101